By Erika J. Ernst
A suite of cutting-edge molecular tools for learning antifungal resistance, for locating and comparing either new and present antifungal medications, and for realizing the host reaction and immunotherapy of such brokers. The protocols stick to the winning equipment in Molecular drugs™ sequence structure, each one providing step by step laboratory directions, an creation outlining the main at the back of the strategy, lists of the mandatory apparatus and reagents, and pointers on troubleshooting and keeping off identified pitfalls. Antifungal brokers: equipment and Protocols deals clinician-scientists, microbiologists and molecular biologists the efficient instruments they want at the present time to appreciate and effectively enhance new healing brokers for yeast, mildew, and fungal infections.
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Extra resources for Antifungal Agents: Methods And Protocols (Methods in Molecular Medicine)
16. Nylon membrane (Nytran Super Charge, Schleicher & Schuell, Dassel, Germany). 17. Vacuum blotter (VacuGene XL, Pharmacia Biotech). 18. Vacuum pump (“VacuGene Pump,” Pharmacia Biotech, Uppsala, Sweden). 19. 3 M sodium citrate). CARE-2 Fingerprinting of C. albicans Isolates 29 Fig. 1. CARE-2 fingerprint pattern of serial Candida albicans isolates obtained from different infection episodes in a single patient. The patient was infected by two different C. albicans strains identifiable by their fingerprint pattern (A vs B).
18. Vacuum pump (“VacuGene Pump,” Pharmacia Biotech, Uppsala, Sweden). 19. 3 M sodium citrate). CARE-2 Fingerprinting of C. albicans Isolates 29 Fig. 1. CARE-2 fingerprint pattern of serial Candida albicans isolates obtained from different infection episodes in a single patient. The patient was infected by two different C. albicans strains identifiable by their fingerprint pattern (A vs B). Subtle differences in the fingerprint pattern are also seen in some isolates of the strain with the B pattern (labeled B1, B2, and B3, and marked by the arrows).
Coke, A. , Burnie, J. , Lucas, G. , and Liu Yin, J. A. (1994) Invasive aspergillosis: clusters and sources? J. Med. Vet. Mycol. 32, 217–224. 18. Meunier, J. , and Grimont, P. A. (1993) Factors affecting reproducibility of random amplified polymorphic DNA fingerprinting. Res. Microbiol. 144, 373–379. CARE-2 Fingerprinting of C. albicans Isolates 27 3 CARE-2 Fingerprinting of Candida albicans Isolates Joachim Morschhäuser, Peter Staib, and Gerwald Köhler Summary The emergence of resistance to antifungal drugs in medically important fungi has become a significant problem in recent years.