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Additional info for Current Topics in Membranes & Transport Vol. 23: Genes & Proteins: Transport Proteins & Receptors
And Adler, J . (1979). Protein methylation in behaviourdl control mechanisms and in signal transduction. Narure (London) 264, 577-579. Wang, E. A , , and Koshland, D. E. (1980). Receptor structure in the bacterial sensing system. Proc. Natl. Acud. Sci. U . S . A . 71, 7157-7161. CURRENT TOPICS IN MEMBRANES AND TRANSPORT. VOLUME 23 Chapter 2 Mutational Analysis of the Structure and Function of the Influenza Virus Hemagg Iut inin MARY-JANE GETHING, CAROLYN DOYLE, MICHAEL ROTH, AND JOE SAMBROOK Cold Spring Harbor Laboratorj Cold Spring Harbor, New York ....
Anchor and cytoplasmic tail of the herpes glycoprotein gpC. This fragment was then inserted in both possible orientations into the BarnHI site of the SV40-HA recombinant vector following the sequences coding for the truncated, anchorminus HA. In one orientation (HA-gC) the open reading frame of HA was continued into the new sequences such that the authentic anchor and tail of gpC replaced those of wild-type HA. In the opposite orientation (HA-Cg), another open reading frame (not thought to be utilized in the herpesvirus genome) is fused to the HA sequences such that the carboxy terminus of the truncated protein is extended by 67 amino acids (mostly polar in nature).
To establish that the protein expressed from the recombinant vector was authentic in its structure, antigenicity, and biological activities, a series of assays was performed including radioimmunoassay , immunoprecipitation, immunofluorescence, and hemagglutination and cell fusion assays. , 1982) and will be discussed only briefly here. , 1982). 1. RADIOIMMUNOASSAY The amounts of Japan HA expressed from the recombinant have been quantitated using a solid-phase radioimmunoassay (Gething and Sambrook, 1981).